L7386

Lysostaphin has endopeptidase activity, which cleaves polyglycine cross-links in the cellular wall of Staphylococcus species. Under certain conditions preparations may also exhibit hexosaminidase activity (specific for the glucosaminyl-muramic acid bond of the bacterial carbohydrate backbone, but does not react with S. aureus) and amidase activity.

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L7386

Sigma

 

Lysostaphin from Staphylococcus staphylolyticus

lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Synonym:Glycyl-glycine Endopeptidase
CAS Number:9011-93-2
Enzyme Commission (EC) Number:3.4.24.75   ( BRENDA | IUBMB )
MDL number:MFCD00131556

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Description

Biochem/physiol ActionsLysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5
Unit DefinitionOne unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Properties

formlyophilized powder
compositionProtein, 50-70% biuret
packagingpkg of (sold on basis of mg protein)
EQP levelPremium
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements22-24/25
WGK Germany3
RTECSOL5985000

References

referenceBoneca, et al., Characterization of Staphylococcus aureus cell wall glycan strands, evidence for a new β-N-acetyl glucosaminidase activity. J. Biol. Chem. 275, 9910-9918, (2000)
 Trayer, H. R., and Buckley, C. E., J. Biol. Chem. 245, 4842-4846 , (1970)
 Browder, H.P., et al., Biochem. Biophys. Res. Commun. 19, 383, (1965)
MerckMerck 13,5659